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Understanding DNA and gene cloning : a guide for the curious / Karl Drlica.

by Drlica, Karl.

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Hoboken, NJ : Wiley, c2004.

Molecular cloning.

Recombinant DNA.

Genetic engineering.

0471434167 (pbk. : alk. paper)

xiv, 369 p. : ill. ; 23 cm.

ch. 1. Preview: Life as interacting molecules -- PART I. BASIC MOLECULAR GENETICS. ch. 2. Structure of DNA: two long, interwound chains -- ch. 3. Reproducing DNA: information transfer from one generation to the next -- ch. 4. Gene expression: cellular use of genetic information -- PART II. MANIPULATING DNA. ch. 5. Microbial growth: one-celled organisms as tools for gene cloning -- ch. 6. Plasmids and phages: submicroscopic parasites used to deliver genes to cells -- ch. 7. Cutting and joining DNA: restriction endonucleases and ligases used to restructure DNA molecules -- ch. 8. Using complementary base pairing: hybridization, probes, and amplification using PCR -- ch. 9. Cloning genes: isolation of a hemoglobin gene -- ch. 10. Using cloned genes: gene structure, expression vectors, and gene function -- PART III. MODERN MOLECULAR GENETICS. ch. 11. Beyond the central dogma: a sampling of insights derived from gene cloning -- ch. 12. Retroviruses: AIDS and gene delivery -- ch. 13. Cancer genes: the loss of growth control -- PART IV: HUMAN GENETICS. ch. 14. Heredity: patterns of inheritance and genetic instability -- ch. 15. Application of human genetics: genetic testing, gene therapy, and DNA fingerprinting -- PART V. WHOLE GENOMES. ch. 16. Genomics: using whole genomes to study life.

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